亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Dynamics of the inhibitory immune checkpoint TIM-3 in mouse pulmonary phagocytes after silica exposure

矽肺 免疫学 免疫系统 巨噬细胞 下调和上调 流式细胞术 吞噬作用 医学 化学 病理 内科学 生物化学 体外 基因
作者
Youliang Zhao,Yaqian Qu,Meixiu Duan,Changfu Hao,Wu Yao
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:261: 115087-115087 被引量:1
标识
DOI:10.1016/j.ecoenv.2023.115087
摘要

Long-term inhalation of silica particles in the workplace causes silicosis, which is incurable and seriously endangers the health of workers. It is believed that silicosis is caused by an imbalance of the pulmonary immune microenvironment, in which pulmonary phagocytes play a crucial role. As an emerging immunomodulatory factor, it is unclear whether T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) participate in silicosis by modulating pulmonary phagocytes function. The purpose of this study was to investigate the dynamic changes of the TIM-3 in pulmonary macrophages, dendritic cells (DCs), and monocytes during the development of silicosis in mice. The plasma levels of soluble TIM-3 in silicosis patients were also examined. Flow cytometry was used to identify alveolar macrophages (AMs), interstitial macrophages (IMs), CD11b+ DC, CD103+ DC, Ly6C+, and Ly6C- monocytes in mouse lung tissues, and further analyses were conducted on the expression of TIM-3. Results showed that soluble TIM-3 was significantly elevated in plasma of silicosis patients, and the level of which was higher in stage II and III patients than that in stage I. In silicosis mice, the protein and mRNA levels of TIM-3 and Galectin9 were significantly upregulated in lung tissues. Specific to pulmonary phagocytes, silica exposure affected TIM-3 expression in a cell-specific and dynamic manner. In macrophages, TIM-3 expression upregulated in AM after 28 days and 56 days of silica instillation, while the expression of TIM-3 in IM decreased at all observation time points. In DCs, silica exposure only caused a decrease of TIM-3 expression in CD11b+ DCs. In monocytes, TIM-3 dynamics in Ly6C+ and Ly6C- monocytes were generally consistent during silicosis development, which significant decrease after 7 and 28 days of silica exposure. In conclusion, TIM-3 may mediate the development of silicosis by regulating pulmonary phagocytes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI

祝大家在新的一年里科研腾飞
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
龚钰茹发布了新的文献求助10
12秒前
科目三应助韩宇航采纳,获得10
12秒前
WWJ完成签到 ,获得积分10
22秒前
34秒前
所所应助沉香续断采纳,获得10
35秒前
动听帆布鞋完成签到 ,获得积分10
49秒前
51秒前
53秒前
DChen完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
1分钟前
XHH完成签到 ,获得积分10
1分钟前
哈哈哈发布了新的文献求助20
2分钟前
2分钟前
2分钟前
YXH发布了新的文献求助10
2分钟前
2分钟前
Hello应助YXH采纳,获得10
2分钟前
行走De太阳花完成签到,获得积分10
2分钟前
杳鸢应助谨慎的炳采纳,获得10
2分钟前
2分钟前
2分钟前
生产队的建设者完成签到,获得积分10
2分钟前
2分钟前
3分钟前
3分钟前
3分钟前
xss完成签到 ,获得积分10
3分钟前
俏皮的安萱完成签到,获得积分10
3分钟前
TEY完成签到 ,获得积分10
3分钟前
SCI的芷蝶完成签到 ,获得积分10
4分钟前
4分钟前
曾经荔枝发布了新的文献求助10
4分钟前
caca完成签到,获得积分10
4分钟前
4分钟前
4分钟前
搜集达人应助曾经荔枝采纳,获得10
4分钟前
HuiHui完成签到,获得积分10
5分钟前
5分钟前
高分求助中
Востребованный временем 2500
Les Mantodea de Guyane 1000
Very-high-order BVD Schemes Using β-variable THINC Method 930
Field Guide to Insects of South Africa 660
The Three Stars Each: The Astrolabes and Related Texts 500
effects of intravenous lidocaine on postoperative pain and gastrointestinal function recovery following gastrointestinal surgery: a meta-analysis 400
The Collected Works of Jeremy Bentham: Rights, Representation, and Reform: Nonsense upon Stilts and Other Writings on the French Revolution 320
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3384373
求助须知:如何正确求助?哪些是违规求助? 2998471
关于积分的说明 8778815
捐赠科研通 2683994
什么是DOI,文献DOI怎么找? 1470088
科研通“疑难数据库(出版商)”最低求助积分说明 679585
邀请新用户注册赠送积分活动 671945