Simultaneous determination of catecholamines, uric acid and ascorbic acid at physiological levels using poly(N-methylpyrrole)/Pd-nanoclusters sensor

抗坏血酸 循环伏安法 电极 介电谱 微分脉冲伏安法 化学 电化学气体传感器 电化学 纳米团簇 电催化剂 玻璃碳 无机化学 分析化学(期刊) 核化学 材料科学 色谱法 有机化学 物理化学 食品科学
作者
Nada F. Atta,Maher F. El‐Kady,Ahmed Galal
出处
期刊:Analytical Biochemistry [Elsevier]
卷期号:400 (1): 78-88 被引量:161
标识
DOI:10.1016/j.ab.2010.01.001
摘要

An interesting electrochemical sensor has been constructed by the electrodeposition of palladium nanoclusters (Pdnano) on poly(N-methylpyrrole) (PMPy) film-coated platinum (Pt) electrode. Cyclic voltammetry, electrochemical impedance spectroscopy (EIS), and scanning electron microscopy were used to characterize the properties of the modified electrode. It was demonstrated that the electroactivity of the modified electrode depends strongly on the electrosynthesis conditions of the PMPy film and Pdnano. Moreover, the modified electrode exhibits strong electrocatalytic activity toward the oxidation of a mixture of dopamine (DA), ascorbic acid (AA), and uric acid (UA) with obvious reduction of overpotentials. The simultaneous analysis of this mixture at conventional (Pt, gold [Au], and glassy carbon) electrodes usually struggles. However, three well-resolved oxidation peaks for AA, DA, and UA with large peak separations allow this modified electrode to individually or simultaneously analyze AA, DA, and UA by using differential pulse voltammetry (DPV) with good stability, sensitivity, and selectivity. This sensor is also ideal for the simultaneous analysis of AA, UA and either of epinephrine (E), norepinephrine (NE) or l-DOPA. Additionally, the sensor shows strong electrocatalytic activity towards acetaminophen (ACOP) and other organic compounds. The calibration curves for AA, DA, and UA were obtained in the ranges of 0.05 to 1 mM, 0.1 to 10 μM, and 0.5 to 20 μM, respectively. The detection limits (signal/noise [S/N] = 3) were 7 μM, 12 nM, and 27 nM for AA, DA, and UA, respectively. The practical application of the modified electrode was demonstrated by measuring the concentrations of AA, DA, and UA in injection sample, human serum, and human urine samples, respectively, with satisfactory results. The reliability and stability of the modified electrode gave a good possibility for applying the technique to routine analysis of AA, DA, and UA in clinical tests.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
无一完成签到 ,获得积分10
刚刚
6秒前
yao完成签到,获得积分10
8秒前
10秒前
11秒前
yao发布了新的文献求助10
11秒前
果果超幼发布了新的文献求助10
14秒前
robi发布了新的文献求助10
15秒前
1592541发布了新的文献求助10
15秒前
打打应助科研通管家采纳,获得10
19秒前
乐乐应助科研通管家采纳,获得10
19秒前
CodeCraft应助科研通管家采纳,获得10
19秒前
科研通AI2S应助科研通管家采纳,获得10
19秒前
大模型应助科研通管家采纳,获得10
19秒前
汉堡包应助科研通管家采纳,获得10
19秒前
共享精神应助科研通管家采纳,获得10
20秒前
科研通AI2S应助科研通管家采纳,获得10
20秒前
葡萄成熟应助zhaoyingxin采纳,获得10
21秒前
我是老大应助zhaoyingxin采纳,获得10
21秒前
22秒前
本之上课完成签到 ,获得积分10
25秒前
1592541完成签到,获得积分20
27秒前
28秒前
不喝奶茶完成签到,获得积分20
28秒前
31秒前
xxxxx发布了新的文献求助10
31秒前
Wang完成签到,获得积分10
32秒前
33秒前
不喝奶茶发布了新的文献求助10
33秒前
坚强亦丝应助hooke采纳,获得10
35秒前
可靠的书桃应助刘斌采纳,获得10
36秒前
liu完成签到 ,获得积分10
36秒前
田様应助健忘溪流采纳,获得10
37秒前
37秒前
41秒前
41秒前
43秒前
本之上课发布了新的文献求助10
44秒前
44秒前
我是老大应助雷声有点响采纳,获得20
46秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Le dégorgement réflexe des Acridiens 800
Defense against predation 800
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3134935
求助须知:如何正确求助?哪些是违规求助? 2785802
关于积分的说明 7774295
捐赠科研通 2441699
什么是DOI,文献DOI怎么找? 1298093
科研通“疑难数据库(出版商)”最低求助积分说明 625075
版权声明 600825