植物乳杆菌
化学
开菲尔
多糖
脱脂牛奶
拉伤
半乳糖
流变学
水溶液
扫描电子显微镜
食品科学
乳酸
细菌
生物化学
有机化学
材料科学
生物
复合材料
解剖
遗传学
作者
Ji Wang,Xiao Zhao,Zheng Tian,Yawei J. Yang,Zhennai Yang
标识
DOI:10.1016/j.carbpol.2015.03.003
摘要
An exopolysaccharide (EPS)-producing strain YW11 isolated from Tibet Kefir was identified as Lactobacillus plantarum, and the strain was shown to produce 90 mg L−1 of EPS when grown in a semi-defined medium. The molecular mass of the EPS was 1.1 × 105 Da. The EPS was composed of glucose and galactose in a molar ratio of 2.71:1, with possible presence of N-acetylated sugar residues in the polysaccharide as confirmed by NMR spectroscopy. Rheological studies showed that the EPS had higher viscosity in skim milk, at lower temperature, or at acidic pH. The viscous nature of the EPS was confirmed by observation with scanning electron microscopy that demonstrated a highly branched and porous structure of the polysaccharide. The atomic force microscopy of the EPS further revealed presence of many spherical lumps, facilitating binding with water in aqueous solution. The EPS had a higher degradation temperature (287.7 °C), suggesting high thermal stability of the EPS.
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