三叉神经节
一氧化氮合酶
免疫印迹
神经营养素
细胞生物学
药理学
生物
神经科学
一氧化氮
化学
医学
内科学
内分泌学
受体
生物化学
感觉系统
基因
作者
Haiqiong Shang,Yan Wang,Xiuhua Chao,Gaoying Sun,Xiaohui Bai,Lei Xu,Yuechen Han,Jianfeng Li,Haibo Wang,Zhaomin Fan
标识
DOI:10.1016/j.neulet.2017.09.016
摘要
Artemin, a member of the glial cell line-derived neurotrophic factor family, is an important cytokine and a critical participant in trigeminal pain disorders such as tongue pain and migraine. However, the mechanisms underlying artemin's activity are largely unknown. In the present study, we used primary cultured trigeminal ganglion neurons (TGNs) to determine the effect of artemin on the expression of the inducible form of nitric oxide synthase (iNOS), which is released in response to painful and inflammatory stimuli. Following artemin treatment, western blot analysis showed that the protein level of iNOS was transiently elevated after artemin treatment for 15min (p<0.05). Immunofluorescence revealed that both the expressions of iNOS and GFRα3 were significantly up-regulated after artemin treatment for 15min. In addition, iNOS expression induced by artemin was co-localized with GFRα3 and TUJ-1 in primary cultured TGNs, respectively. Our results indicate a previously unknown role of artemin in regulating iNOS expression in primary cultured TGNs, and regulation of iNOS might be involved in the mechanism through which artemin participates in the trigeminal pain pathway.
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