神经突
脊髓损伤
神经科学
细胞生物学
胶质瘢痕
轴突引导
坐骨神经
体内
作者
Jose Gerardo-Nava,Dorothee Hodde,Istvan Katona,Ahmet Bozkurt,Torsten Grehl,Harry W.M. Steinbusch,Joachim Weis,Gary A. Brook
出处
期刊:Biomaterials
[Elsevier]
日期:2014-05-01
卷期号:35 (14): 4288-4296
被引量:32
标识
DOI:10.1016/j.biomaterials.2014.02.007
摘要
Numerous in-vitro techniques exist for investigating the influence of 3D substrate topography on sensory axon growth. However, simple and cost-effective methods for studying post-natal motor axon interactions with such substrates are lacking. Here, spinal cord organotypic slice cultures (OSC) from post-natal day 7–9 rat pups were presented with spinal nerve roots, or blocks of fibrin hydrogel or 3D microporous collagen scaffolds to investigate motor axon–substrate interactions. By 7–14 days, axons from motor neuronal pools extended into the explanted nerve roots, growing along Schwann cell processes and demonstrating a full range of axon-Schwann cell interactions, from simple ensheathment to concentric wrapping by Schwann cell processes and the formation of compact myelin within a basal lamina sheath. Extensive motor axon regeneration and all stages of axon-Schwann interactions were also supported within the longitudinally orientated microporous framework of the 3D collagen scaffold. In stark contrast, the simple fibrin hydrogel only supported axon growth and cell migration over its surface. The relative ease of demonstrating such motor axon regeneration through the microporous 3D framework by immunofluorescence, two-photon microscopy and transmission electron microscopy strongly supports the adoption of this technique for assaying the influence of substrate topography and functionalization in regenerative bioengineering.
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