Direct carbon capture for production of high-performance biodegradable plastic by cyanobacterial cell factory

羟基烷酸 代谢工程 聚乳酸 生物可分解塑胶 生物塑料 原材料 钩虫贪铜菌 食品科学 异养 聚羟基丁酸酯 化学 生物化学 废物管理 生物 有机化学 细菌 聚合物 工程类 遗传学
作者
Chunlin Tan,Fei Tao,Ping Xu
标识
DOI:10.1101/2021.10.04.462501
摘要

Abstract Plastic pollution has become one of the most pressing environmental issues today, leading to an urgent need to develop biodegradable plastics 1-3 . Polylactic acid (PLA) is one of the most promising biodegradable materials because of its potential applications in disposable packaging, agriculture, medicine, and printing filaments for 3D printers 4-6 . However, current biosynthesis of PLA entirely uses edible biomass as feedstock, which leads to competition for resources between material production and food supply 7,8 . Meanwhile, excessive emission of CO 2 that is the most abundant carbon source aggravates global warming, and climate instability. Herein, we first developed a cyanobacterial cell factory for the de novo biosynthesis of PLA directly from CO 2 , using a combinational strategy of metabolic engineering and high-density cultivation (HDC). Firstly, the heterologous pathway for PLA production, which involves engineered D-lactic dehydrogenase (LDH), propionate CoA-transferase (PCT), and polyhydroxyalkanoate (PHA) synthase, was introduced into Synechococcus elongatus PCC7942. Subsequently, different metabolic engineering strategies, including pathway debottlenecking, acetyl-CoA self-circulation, and carbon-flux redirection, were systematically applied, resulting in approximately 19-fold increase to 15 mg/g dry cell weight (DCW) PLA compared to the control. In addition, HDC increased cell density by 10-fold. Finally, the PLA titer of 108 mg/L (corresponding to 23 mg/g DCW) was obtained, approximately 270 times higher than that obtained from the initially constructed strain. Moreover, molecular weight (M w , 62.5 kDa; M n , 32.8 kDa) of PLA produced by this strategy was among the highest reported levels. This study sheds a bright light on the prospects of plastic production from CO 2 using cyanobacterial cell factories.
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