铜绿假单胞菌
抗菌剂
锌
广谱
化学
纳米颗粒
微生物学
核化学
纳米技术
材料科学
有机化学
细菌
生物
组合化学
遗传学
作者
Manjari Barsainya,Devendra P. Singh
标识
DOI:10.22207/jpam.12.4.50
摘要
The present work highlights a low-cost, ecofriendly and simple procedure for the synthesis of zinc oxide nanoparticles (ZnONPs), mediated by extracellular secondary metabolite pyoverdine -a siderophore produced by Pseudomonas aeruginosa.The in vitro bio-reduction of zinc nitrate into ZnONPs in the presence of siderophore pyoverdine was ascertained by UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy and transmission electron microscopy (TEM) analysis.The synthesized ZnONPs were distinguished by a characteristic absorption peak at 416 nm in the UV-visible range.The TEM pictures of ZnONPs showed pseudospherical morphology, with particle size ranging between 50 and 100 nm.X-ray diffraction peaks obtained at 31.73 °, 34.37 °, 36.24°, 47.40 °, 56.68 °, 63.0 ° and 67.8 ° corresponding to the lattice plane of [100], [002], [101], [102], [110], [103] and [112] suggested the polycrystalline wurtzite structure of the nanoparticle.The ZnONPs were found to have an antibacterial effect against Gram-positive bacteria (Staphylococcus aureus and Bacillus sp.) and Gram-negative bacteria (Escherichia coli), and also showed a fungicidal effect against phytopathogenic fungi such as Rhizoctonia solani, Fusarium sp. and Penicillium sp.The present approach of using exogenously produced bacterial siderophore offers a unique in vitro green method of synthesizing ZnONPs, where a direct contact between the toxic metal and biological resource material can be avoided.
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