肺癌
医学
癌症
肺
病理
转移
癌细胞
免疫组织化学
刘易斯肺癌
动物模型
癌症研究
内科学
作者
Nensi Shrestha,Zabeen Lateef,Orleans Martey,Abigail R. Bland,Mhairi Nimick,Rhonda J. Rosengren,John C. Ashton
出处
期刊:F1000Research
[F1000 Research Ltd]
日期:2019-02-15
卷期号:8: 190-190
被引量:7
标识
DOI:10.12688/f1000research.17964.1
摘要
Lung cancer drug development requires screening in animal models. We aimed to develop orthotopic models of human non-small lung cancer using A549 and H3122 cells delivered by tail vein injection. This procedure has been used previously for a mouse lung cancer (Lewis lung carcinoma) and as a model of human breast cancer metastasis to lung. We report that the procedure led to poor animal condition 7-8 weeks after injection, and produced lesions in the lungs visible at necropsy but we were unable identify individual cancer cells using immunohistochemistry. We conclude that if this method is to produce a model that can be used in drug experiments, improvements are required for cancer cell detection post mortem, such as by using of a fluorescently tagged human lung cancer cell line.
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