Chemoenzymatic synthesis of the oligosaccharide moiety of the tumor-associated antigen disialosyl globopentaosylceramide

The oligosaccharide of the tumor-associated antigen DSGb5 was synthesized in a chemoenzymatic manner by exploiting the mammalian glycosyl transferases ST3Gal1 and ST6GalNAc5, and its binding with Siglec-7 was investigated by glycan microarray technology.