荧光团
罗丹明
荧光
生物成像
亚历山福禄
罗丹明B
近红外光谱
化学
材料科学
纳米技术
有机化学
光学
光催化
物理
催化作用
作者
Jonathan B. Grimm,Ariana N. Tkachuk,Liangqi Xie,Heejun Choi,Boaz Mohar,Natalie Falco,Kathy Schaefer,Ronak Patel,Qinsi Zheng,Zhe Liu,Jennifer Lippincott‐Schwartz,Timothy A. Brown,Luke D. Lavis
出处
期刊:Nature Methods
[Springer Nature]
日期:2020-07-27
卷期号:17 (8): 815-821
被引量:179
标识
DOI:10.1038/s41592-020-0909-6
摘要
Expanding the palette of fluorescent dyes is vital to push the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore owing to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance due to their propensity to adopt a lipophilic, nonfluorescent form. We report a framework for rationalizing rhodamine behavior in biological environments and a general chemical modification for rhodamines that optimizes long-wavelength variants and enables facile functionalization with different chemical groups. This strategy yields red-shifted 'Janelia Fluor' (JF) dyes useful for biological imaging experiments in cells and in vivo.
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