杜氏利什曼原虫
免疫系统
免疫学
巨噬细胞
利什曼原虫
生物
利什曼原虫
效应器
白细胞介素10
抗原
细胞生物学
利什曼病
内脏利什曼病
体外
万维网
寄生虫寄主
生物化学
计算机科学
作者
Arun Kumar Rawat,Kavita Pal,Rajan Singh,Anshul Anand,Saurabh Gupta,Dhiraj Kishore,Sangram Singh,Rakesh Kumar Singh
标识
DOI:10.1016/j.ijbiomac.2020.02.189
摘要
The lacuna in the knowledge of immunobiology, especially in visceral infections that are fatal if left untreated, are a major hurdle in getting a vaccine candidate for leishmaniasis. Till date, only a few drugs are available to combat human leishmaniasis and a vaccine candidate either prophylactic or preventive is still awaited. Therefore, identification of host and parasitic factors involved in the regulation of specific immune mechanisms are essentially needed. In this study, we observed that CD200-CD200R immune inhibitory axis regulates host macrophages effectors properties and helps antigen experienced T cells (CD4+CD44+ T cells) to acquire anti-inflammatory cytokines (IL-4, IL-10, TGF-β, IL-27) producing abilities in an NFkB independent manner. After CD200 blocking the macrophages effectively inhibited proliferation of Leishmania amastigotes and also induced the production of IL-12, IFN-γ, TNF-α and nitric oxide (NOx). Further, the blocking of CD200 signaling also restored macrophages MHC-II expression and helped CD4+CD44+ T cells to produce pro-inflammatory cytokines like IL-2, IL-12 and IFN-γ. The finding of this study suggested the importance of immune inhibitory mechanisms in controlling Leishmania growth and survival and therefore, requires more studies to understand its role in vaccine induced immunity.
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