Enzymatic preparation of structured TAG containing conjugated linoleic acid (CLA) at solvent-free

Abstract Rice bran oil extracted by the water-enzymatic method was placed at a temperature of −5 °C for 8 h. Light-phase rice bran oil with an iodine value of 112.13 ± 0.21 g I 2 /100 g oil was separated by cryogenic freezing centrifugation. Lipozyme RM IM transesterified light-phase rice bran oil and conjugated linoleic acid (CLA) under supercritical system CO 2 (SC-CO 2 ). The optimal reaction conditions for transesterification of CLA and rice bran oil in SC-CO 2 were determined as follows: the Lipozyme RM IM dosage was 9%, and the RBO/CLA molar ratio was 1:3, the reaction temperature was 55 °C, stirring speed was 300 rpm, and transesterification time was 20 h, the CLA conversion rate can reach 42.1%.