吞噬作用
流式细胞术
巨噬细胞
先天免疫系统
吞噬细胞
细胞生物学
荧光显微镜
免疫系统
生物
微生物学
传出细胞增多
免疫学
化学
荧光
体外
生物化学
物理
量子力学
作者
Lokesh Sharma,Wenjun Wu,Sanjay L. Dholakiya,Samir Gorasiya,Jiao Wu,Ravikumar Sitapara,Vivek Patel,Mao Wang,Michelle Zur,Shloka Reddy,Nathan Siegelaub,Katrina Bamba,Frank A. Barile,Lin L. Mantell
出处
期刊:Methods in molecular biology
日期:2014-01-01
卷期号:: 137-145
被引量:27
标识
DOI:10.1007/978-1-4939-0928-5_12
摘要
Phagocytosis is the process by which phagocytes, including macrophages, neutrophils and monocytes, engulf and kill invading pathogens, remove foreign particles, and clear cell debris. Phagocytes and their ability to phagocytose are an important part of the innate immune system and are critical for homeostasis of the host. Impairment in phagocytosis has been associated with numerous diseases and disorders. Different cytokines have been shown to affect the phagocytic process. Cytokines including TNFα, IL-1β, GM-CSF, and TGF-β1 were found to promote phagocytosis, whereas high mobility group box-1 (HMGB1) inhibited the phagocytic function of macrophages. Here, we describe two commonly used methods to assess the phagocytic function of cultured macrophages, which can easily be applied to other phagocytes. Each method is based on the extent of engulfment of FITC-labeled latex minibeads by macrophages under different conditions. Phagocytic activity can be assessed either by counting individual cells using a fluorescence microscope or measuring fluorescence intensity using a flow cytometer.
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