光学相干层析成像
离体
体内
视网膜
生物医学工程
视网膜
临床前影像学
眼科
病理
医学
生物
神经科学
生物技术
作者
Sven Schnichels,Tanja Dorfi,Maximilian Schultheiß,Marius Ueffing,Karl-Ulrich Bartz-Schmidt,Kai Januschowski,Martin S. Spitzer,Focke Ziemssen
标识
DOI:10.1016/j.exer.2016.04.011
摘要
Optical coherence tomography (OCT) dramatically changed the way of diagnostic assessment in retinal diseases during the last years. Using this technique in-vivo in-depth analysis of the retina and its layers is possible. Since animal research is changing by intrinsic and extrinsic pressure to animal-(in-vivo)-free methods, we adapted OCT-measurements to organotypic cultures. An easy to use protocol was generated to assess standardized OCT assessments in organotypic culture. First, two custom-made devices need to be made to change any commercially available OCT for examinations in humans into a device allowing ex-vivo analyses of organotypic culture. The modification is feasible within seconds. After OCT measurement of the ex-vivo tissues, quantitative evaluation of the retinas were performed via ImageJ software. OCT pictures of ex-vivo retinas were obtained for time periods of seven days and the thickness of retinal tissue was evaluated. The reproducibility of the pictures and measurements was very high (SD < 15%). In conclusion, an easy to use protocol for the investigation of different effects on retinal cultures with commercially available OCT devices was successfully established.
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