Influence of colchicine on radicle growth of Vicia fabaand the polyploid inductive effect

In order to test the optimum induction combination(time and concentration),and analyse the inductive effects of colchicine induced the radicle polyploid in Vicia faba.The radicle cells were treated with the colchicine of five concentrations(0.025%,0.050%,0.100%,0.150%,0.200%,respectively) combining various culture time(12,24,48,60h,respectively).And the radicle cell were treated with distilled water simultaneously,which were taken as the control.The optimum induction combination induced by colchicine was confirmed by significant difference of the root tip inductivity and the radicle expanding ratio.And the chromosome number of radicle cells and stoma number of seedling were detected to identify the induction effect.The radicle amount and seeding height were analized subsequently.The optimum inductive condition was 48hcombining colchicine concentration of 0.100%.There were significant differences in stoma number between inducted time and inducted concentration,however,the radicle number and height of V.fabaseedling showed significant difference in inductive concentration but no significant difference in culture time.