农杆菌
转化(遗传学)
龙葵
绿色荧光蛋白
生物
基因
表达式向量
瞬态(计算机编程)
基因表达
重组DNA
接种
细胞生物学
生物技术
植物
园艺
遗传学
计算机科学
操作系统
作者
Liping Yang,Taicheng Jin,Hongwei Xu,Hua Li,Xiaofu Zhou
出处
期刊:Yichuan
[China Science Publishing & Media Ltd.]
日期:2013-09-27
卷期号:35 (1): 111-117
被引量:1
标识
DOI:10.3724/sp.j.1005.2013.00111
摘要
Due to the laborious and scale-up limitation we have developed a simple method named "seed absorption" to express foreign proteins in plants by means of transient expression. It has been shown that the reporter gene GFP was expressed successfully in tomato (Solanum lycopersicum L.) plants by seed absorbing Agrobacterium suspension containing TMV-based p35S-30B-GFP vector. Various factors influencing the gene expression were optimized including Agrobacterium cell density and other inoculation conditions. This method has the special advantages as simple work process, ease to scale-up, and further expanding the host range of plant bioreactor than previous methods. We assume that the seed absorption method will facilitate the industrial scale production of the recombinant pharmaceutical proteins in plants.
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