BAF_Protocol_002 On-Bead Digestion (Magnetic Beads) of Proteins in Solution or Provided Already on Beads v1

This protocol is used to more easily digest a tissue or cell protein lysate (after precipitation and reconstitution) on a magnetic bead for easier manipulation and washing. The protocol from step 13 on would be used for proteins already captured on a magnetic beads such as antibody (IP) or avidin coated - usually as an enrichment step. The proteins are easily washed to remove contaminants such as detergents or salts. The resulting peptide mixture can be further purified using a C18 tip such as our Protocol 003.