化学
粒体自噬
荧光
吲哚试验
活力测定
缺氧(环境)
酰胺
细胞器
细胞
线粒体
生物化学
癌细胞
分子生物学
生物物理学
细胞凋亡
癌症
氧气
自噬
生物
有机化学
物理
量子力学
遗传学
作者
Sirilak Wangngae,Kantapat Chansaenpak,Tunyawat Khrootkaew,Rung‐Yi Lai,Anyanee Kamkaew
标识
DOI:10.1016/j.bioorg.2022.106173
摘要
As pH-sensitive and hypoxia-responsive probes, three hemicyanine derivatives based on vanillin and the indole ring (Val-Hcys) were synthesized. The fluorescence of the probes can be activated at acidic pH using the amide functionalized sidechains. Furthermore, when Val-Hcys were incubated with hypoxic cells for 5 min, the fluorescent signals significantly increased when compared to normoxia cells (4-fold enhancement, maximum at 180 min). In addition, Val-Hcys tend to accumulate in lysosomes and mitochondria, two important organelles involved in cell mitophagy. Surprisingly, Val-Hcys improved cell viability in hypoxic conditions. As a result, this study demonstrates the utility of Val-Hcys as pH-responsive probes for detecting hypoxic areas.
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