光形态发生
磷酸化
拟南芥
细胞生物学
下胚轴
光敏色素
生物
激酶
拟南芥
基因
生物化学
植物
突变体
红灯
作者
Deepika Sharma,Dhanraj Singh,Kirti Singh,Aditi Dwivedi,Aashish Ranjan,Alok Krishna Sinha
标识
DOI:10.1016/j.envexpbot.2023.105345
摘要
Light is one of the most important environmental factors that affect overall growth and development in plants. PHYTOCHROME INTERACTING FACTORs (PIFs) are negative regulators of photomorphogenesis. PIFs mediate light responses by interacting with downstream molecular partners and are essential regulators for hypocotyl elongation in Arabidopsis. Light induce activation of phytochromes (phys), promotes rapid phosphorylation, ubiquitination and degradation of PIFs. However the kinase responsible for the phosphorylation of PIFs and the signaling mechanism governing the adaptive changes leading to hypocotyl elongation to differential light intensities is not well understood. Here, we report interaction and phosphorylation of PIF3 by a mitogen-activated protein kinase 6 (MPK6) both in vitro and in vivo. Phosphorylation was significantly abolished when all the PIF3 putative phosphorylation sites were mutated to alanine. Further, we found that PIF3 directly binds to the bHLH binding domain in the promoter of RL responsive miRNA, miR163. Interestingly, phosphorylation status of PIF3 substantially affects the binding of PIF3 and mutated proteins to miR163 promoter. Further, overexpression of PIF3 affects the expression of red light (RL) responsive miRNAs and downstream genes involved in hypocotyl elongation and seedling development. These results suggest that MPK6-PIF3 module functions upstream of RL-responsive miRNAs and PIF3-regulated genes involved in photomorphogenesis, thus interconnecting these pathways together.
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