Peripheral blood mononuclear cell- transcriptome signatures of atopic dermatitis and prediction for the efficacy of dupilumab

杜皮鲁玛 转录组 外周血单个核细胞 特应性皮炎 免疫学 医学 免疫系统 湿疹面积及严重程度指数 基因表达 基因 生物 内科学 遗传学 体外
作者
Yu Wang,Yuemeng Wu,Chaoying Gu,Shangshang Wang,Huibin Yin,Ronghui Zhu,Ce Wang,Zheng Li,Xu Yao,Wei Li
出处
期刊:Journal of Dermatological Science [Elsevier BV]
卷期号:111 (3): 83-92 被引量:4
标识
DOI:10.1016/j.jdermsci.2023.06.002
摘要

Few studies have explored transcriptome of the peripheral blood mononuclear cells (PBMCs) of atopic dermatitis (AD). Parameters for prediction of the efficacy of dupilumab in AD remain obscure.To explore transcriptome signature of the PBMCs from Chinese AD patients and the usage in predication for the efficacy of dupilumab.A total of 56 moderate-to-severe adult AD patients were enrolled and followed up for 16 week-dupilumab treatment. PBMCs samples were collected at baseline and 16 weeks after dupilumab treatment. Thirty-five patients were subjected to RNA-sequencing. Weighted gene co-expression network analysis (WGCNA) was used to find genes for prediction of dupilumab efficacy, which was validated in the rest 21 AD patients. Another 30 healthy individuals were enrolled and subjected to RNA-sequencing as healthy controls.Upregulation of the T helper (Th) 2/Th22 pathway, Th17 antimicrobial genes, and natural T-regulatory cell abundance in the PBMCs of AD cases was observed, whereas TGF-β signaling and NK-cell signaling were decreased. Dupilumab treatment reversed the increase in the expression of Th2 cytokine receptors. WGCNA identified two immune-related modules that were correlated significantly with the efficacy of dupilumab. Hub gene MAP2K3 and UBE2L3 of these two modules demonstrated potential predictive ability for efficacy in the RNA-sequencing group by Spearman correlation, ROC analysis, and regression analysis, which was further validated in additional 21 AD cases.We firstly revealed the molecular phenotype of PBMCs in Chinese patients with AD, and uncovered two molecules that might be useful for prediction of the efficacy of dupilumab.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
英俊的铭应助wellyou采纳,获得10
刚刚
风中的向卉完成签到 ,获得积分10
3秒前
Mp4完成签到 ,获得积分10
3秒前
凌兰完成签到 ,获得积分10
3秒前
plain完成签到,获得积分10
4秒前
陌上花开完成签到,获得积分10
5秒前
6秒前
fg2477完成签到,获得积分10
7秒前
忙碌的数学人完成签到,获得积分10
7秒前
情怀应助Engen采纳,获得10
7秒前
HJJHJH完成签到,获得积分10
9秒前
Bob发布了新的文献求助10
10秒前
11秒前
12秒前
HJJHJH发布了新的文献求助50
13秒前
JW完成签到,获得积分10
13秒前
wanci应助张参采纳,获得10
14秒前
谦让的西装完成签到 ,获得积分10
15秒前
李演员完成签到,获得积分10
16秒前
fei菲飞完成签到,获得积分10
16秒前
18秒前
Zhaowx完成签到,获得积分10
18秒前
Theprisoners完成签到,获得积分0
18秒前
木子发布了新的文献求助30
18秒前
18秒前
下课了吧完成签到,获得积分10
19秒前
丘比特应助xialuoke采纳,获得10
20秒前
zgt01发布了新的文献求助10
22秒前
linfordlu完成签到,获得积分0
22秒前
清浅发布了新的文献求助10
23秒前
风趣的涵柏完成签到,获得积分10
24秒前
26秒前
Chen完成签到 ,获得积分10
27秒前
28秒前
木樨完成签到,获得积分10
29秒前
科研顺利完成签到,获得积分10
30秒前
Bin完成签到,获得积分10
31秒前
gszy1975发布了新的文献求助10
31秒前
十曰发布了新的文献求助10
32秒前
33秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038235
求助须知:如何正确求助?哪些是违规求助? 3575992
关于积分的说明 11374009
捐赠科研通 3305760
什么是DOI,文献DOI怎么找? 1819276
邀请新用户注册赠送积分活动 892662
科研通“疑难数据库(出版商)”最低求助积分说明 815022