Aided Porous Medium Emulsification for Functional Hydrogel Microparticles Synthesis

Abstract Despite the substantial advancement in developing various hydrogel microparticle (HMP) synthesis methods, emulsification through porous medium to synthesize functional hybrid protein–polymer HMPs has yet to be addressed. Here, the aided porous medium emulsification for hydrogel microparticle synthesis (APME‐HMS) system, an innovative approach drawing inspiration from porous medium emulsification is introduced. This method capitalizes on emulsifying immiscible phases within a 3D porous structure for optimal HMP production. Using the APME‐HMS system, synthesized responsive bovine serum albumin (BSA) and polyethylene glycol diacrylate (PEGDA) HMPs of various sizes are successfully synthesized. Preserving protein structural integrity and functionality enable the formation of cytochrome c (cyt c) – PEGDA HMPs for hydrogen peroxide (H 2 O 2 ) detection at various concentrations. The flexibility of the APME‐HMS system is demonstrated by its ability to efficiently synthesize HMPs using low volumes (≈50 µL) and concentrations (100 µ m ) of proteins within minutes while preserving proteins’ structural and functional properties. Additionally, the capability of the APME‐HMS method to produce a diverse array of HMP types enriches the palette of HMP fabrication techniques, presenting it as a cost‐effective, biocompatible, and scalable alternative for various biomedical applications, such as controlled drug delivery, 3D printing bio‐inks, biosensing devices, with potential implications even in culinary applications.