亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Synergistic antimicrobial combination of third-generation cephalosporins and polymyxin B against carbapenem-polymyxin-resistant Klebsiella pneumoniae: an in vitro and in vivo analysis

肺炎克雷伯菌 体内 微生物学 多粘菌素B 溶血 头孢菌素 多粘菌素 头孢噻肟 最小抑制浓度 抗生素 碳青霉烯 生物 化学 大肠杆菌 免疫学 生物化学 基因 生物技术
作者
Mariana Carvalho Sturaro,Nathalia da Silva Damaceno,Izadora Dillis Faccin,Osmar N. Silva,Thiago Mendonça de Aquino,Nathalia Monteiro Lins Freire,Marcone Gomes dos Santos Alcântara,Kadja Luana Chagas Monteiro,Luana Rossato,Gleyce Hellen de Almeida de Souza,Simone Simionatto
出处
期刊:Antimicrobial Agents and Chemotherapy [American Society for Microbiology]
标识
DOI:10.1128/aac.00930-24
摘要

ABSTRACT Antibiotic combination therapy is a promising approach to address the urgent need for novel treatment options for infections caused by carbapenem-polymyxin-resistant Klebsiella pneumoniae (CPR-Kp). The present study aimed to investigate the synergistic potential of four cephalosporins in combination with polymyxin B (PMB). A checkerboard assay was performed to evaluate the synergistic effects of cephalexin (CLX), cefixime, cefotaxime (CTX), and cefmenoxime (CMX) in combination with PMB. Subsequently, experiments evaluating the use of CTX or CMX in combination with PMB (CTX-PMB or CMX-PMB, respectively), including growth curve and SynergyFinder analysis, antibiofilm activity assays, cell membrane integrity assays, and scanning electron microscopy, were performed. Safety assessments were also conducted, including hemolysis and toxicity evaluations, using Caenorhabditis elegans . Furthermore, an in vivo model in C. elegans was adopted to assess the treatment efficacy against CPR-Kp infections. CTX-PMB and CMX-PMB exhibited low fractional inhibitory concentration indexes ranging from 0.19 to 0.50 and from 0.25 to 1.5, respectively, and zero interaction potency scores of 37.484 and 15.076, respectively. The two combinations significantly reduced growth and biofilm formation in CPR-Kp. Neither CTX-PMB nor CMX-PMB compromised bacterial cell integrity. Safety assessments revealed a low hemolysis percentage and high survival rates in the C. elegans toxicity evaluations. The in vivo model revealed that the CTX-PMB and CMX-PMB treatments improved the survival rates of C. elegans . The synergistic effects of the CTX-PMB and CMX-PMB combinations, both in vitro and in vivo , indicate that these antibiotic pairings could represent effective therapeutic options for infections caused by CPR-Kp.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小蘑菇应助鲜于元龙采纳,获得10
19秒前
30秒前
StayGolDay完成签到,获得积分10
38秒前
41秒前
英喆完成签到 ,获得积分10
43秒前
科研通AI2S应助科研通管家采纳,获得10
45秒前
迅速灵竹完成签到 ,获得积分10
49秒前
51秒前
鲜于元龙发布了新的文献求助10
55秒前
1分钟前
CASLSD完成签到 ,获得积分10
1分钟前
gwbk完成签到,获得积分10
1分钟前
1分钟前
传奇3应助鲜于元龙采纳,获得10
1分钟前
1分钟前
1分钟前
幽默涵易发布了新的文献求助10
1分钟前
在水一方应助nhh采纳,获得10
2分钟前
nhh给nhh的求助进行了留言
2分钟前
2分钟前
2分钟前
xdjack发布了新的文献求助10
2分钟前
2分钟前
鲜于元龙发布了新的文献求助10
2分钟前
略略略发布了新的文献求助10
2分钟前
2分钟前
爱静静完成签到,获得积分0
2分钟前
汉堡包应助科研通管家采纳,获得10
2分钟前
hahahan完成签到 ,获得积分10
2分钟前
李健的粉丝团团长应助xun采纳,获得10
2分钟前
2分钟前
xdjack发布了新的文献求助10
2分钟前
nhh给nhh的求助进行了留言
2分钟前
3分钟前
3分钟前
3分钟前
Jack发布了新的文献求助10
3分钟前
隐形曼青应助言言言言采纳,获得10
3分钟前
xun发布了新的文献求助10
3分钟前
ding应助Jack采纳,获得10
3分钟前
高分求助中
Solution Manual for Strategic Compensation A Human Resource Management Approach 1200
Natural History of Mantodea 螳螂的自然史 1000
Glucuronolactone Market Outlook Report: Industry Size, Competition, Trends and Growth Opportunities by Region, YoY Forecasts from 2024 to 2031 800
A Photographic Guide to Mantis of China 常见螳螂野外识别手册 800
Autoregulatory progressive resistance exercise: linear versus a velocity-based flexible model 500
The analysis and solution of partial differential equations 400
Spatial Political Economy: Uneven Development and the Production of Nature in Chile 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3335359
求助须知:如何正确求助?哪些是违规求助? 2964501
关于积分的说明 8614028
捐赠科研通 2643363
什么是DOI,文献DOI怎么找? 1447420
科研通“疑难数据库(出版商)”最低求助积分说明 670597
邀请新用户注册赠送积分活动 658974