吸光度
比色法
比色分析
催化作用
检出限
基质(水族馆)
选择性
材料科学
焦磷酸盐
组合化学
化学
线性范围
色谱法
生物传感器
纳米技术
生物化学
酶
海洋学
地质学
作者
Haoyu Yang,Zengxu Liu,Chengzhen Liu,Yanan Zhang
摘要
Assays of pyrophosphate ion (PPi) are of remarkable biochemical significance due to their vital roles in the bioenergetic and metabolic processes or as disease indicators. Colorimetry is popular in the field of biosensing and detection because of its simplicity, speed and cost-effectiveness, but there is a lack of a suitable colorimetric probe. Herein, a novel colorimetric sensing platform has been established for the detection of pyrophosphate based on the FeMoO4-H2O2-3,3',5,5'-tetra-methylbenzidine (TMB) system. Compared with most previously reported iron-based nanozymes, the as-obtained FeMoO4 nanospheres with a rough surface possessed a much superior peroxidase-like catalytic activity (Vmax = 28.47 × 10-8 M s-1) and substrate affinity (Km = 0.174 mM) toward H2O2 catalysis. Due to the Fe(II) and PPi reaction, the presence of PPi could specifically restore blue oxidized TMB to colorless TMB, which led to a decrease in UV absorption at 652 nm. The absorbance change is proportional to the PPi concentration, with a linear detection range (from 0.5 to 25 μM) and a low detection limit of 0.3 μM (S/N = 3). Accordingly, its excellent selectivity and high sensitivity made it a potential colorimetric sensor for PPi analysis in actual water samples.
科研通智能强力驱动
Strongly Powered by AbleSci AI