Long-Term Cryopreservation of Ready-to-Use DNA-Modified Gold Nanoparticle Derivatives: Effect of Preservation Temperature on Their DNA Dissociation and Functional Stability

Abstract The effect of preservation temperature on the DNA dissociation and functional stability of two types of DNA-modified gold nanoparticles (GNPs) was studied. The cryopreservation of both GNPs for 56 days has fully prevented the DNA dissociation and maintained the toehold-mediated DNA displacement reaction capacity without any aggregation of GNPs.