Long term application of plant growth-promoting bacterium improved grain weight and reduced arsenic accumulation in rice grain: A comparison of 10 bacteria

Rice (Oryza sativa L.) is one of the main food crops, it plays an important role in the human diet. Arsenic (As) contamination in paddy soil inhibits rice growth and reduces rice yield seriously. In addition, As accumulated in rice grains was harmful to human health through the food chain. Using the exogenous method to alleviate As stress and reduce As accumulation in rice grain is one of the potential ways to achieve food safety in polluted farmland. In the present study, 10 bacteria was applied to evaluate the effects of plant growth-promoting bacteria (PGPBs) on rice growth and As accumulation in rice grain. The results showed higher levels of As inhibited PGPB growth, the most tolerant and sensitive bacteria were Bj05 and Ls09, with the growth reduction of 16.9% and 96.7% under 50 mM As, respectively. Most of 10 PGPBs enhanced rice growth and improved rice grain weight under As exposure, among them, Ts06 showed the most effective one. Six of 10 PGPBs reduced rice grain As levels significantly, the highest reduction of grain As was observed in Ts06 inoculated rice, with grain As deceasing to 46.3% of the control. Bj05 was the only one which caused the increase in grain As of Yangdao 6. The Pearson correlation analysis showed grain As concentration negatively correlated with leave As concentration, while did not correlated with total As accumulated in shoot, and soil available As and P. The present results indicated that some PGPBs inhibited As translocation from leave to grain, thus reduced As accumulation in rice grain. Ts06 was suggested to be a candidate as microbial amendments for As-contaminated paddy fields.