Establishment of measurement system of ginsenoside Rh2 glycosyltransferase activity.

[Objective] The aim was to establish the activity assay system of uridine 5'-diphosphoglucose(UDPG):ginsenoside Rh2 glucosyltransferase(UGRh2GT) of Panax ginseng cells.[Method] With UDPG as the glucose donor,ginsenoside Rh2 was catalyzed into Rg3 by certain enzyme which was extracted from P.ginseng suspension cells at appropriate temperature and pH.The generated ginsenoside Rg3 was determined by high performance liquid chromatography (HPLC).The analytical column was C18 (150 mm×4.6 mm).Mobile phases were acetonitrile and water with gradient elution.[Result] In the activity assay system of UGRh2GT of ginseng cells,the optimum pH value was 9.3,the optimal temperature was 34.1℃.[Conclusion] The method of activity assay can determine the activity of UGRh2GT more easily.