标题 |
Characterization of PNA and DNA Immobilization and Subsequent Hybridization with DNA Using Acoustic-Shear-Wave Attenuation Measurements
使用声剪切波衰减测量表征PNA和DNA固定以及随后与DNA的杂交
相关领域
肽核酸
石英晶体微天平
化学
生物素化
DNA
生物素
链霉亲和素
寡核苷酸
核酸热力学
核酸
生物物理学
生物化学
有机化学
生物
吸附
基序列
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DOI |
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其它 | We report here how the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, simultaneously measuring changes in the induced energy dissipation, D (cf. viscoelastic properties), and the frequency, f (cf. coupled mass), can be used to characterize the bound state of single-stranded peptide nucleic acid (PNA) and deoxyribose nucleic acid (DNA) in relation to their ability to function as selective probe(s) for fully complementary and single-mismatch DNA. The possibility to use the QCM-D technique for detection of binding kinetics and structural differences in the formed duplexes is also presented. |
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