| 标题 |
 DNA-Barcoded Plasmonic Nanostructures for Activity-Based Protease Sensing DNA条形码等离子体纳米结构用于基于活性的蛋白酶传感
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| 网址 | |
| DOI | |
| 其它 | We report the development of a new class of protease activity sensors called DNA-barcoded plasmonic nanostructures. These probes are comprised of gold nanoparticles functionalized with peptide-DNA conjugates (GPDs), where the peptide is a substrate of the protease of interest. The DNA acts as a barcode identifying the peptide and facilitates signal amplification. Protease-mediated peptide cleavage frees the DNA from the nanoparticle surface, which is subsequently measured via a CRISPR/Cas12a-based assay as a proxy for protease activity. As proof-of-concept, we show activity-based, multiplexed detection of the SARS-CoV-2-associated protease, 3CL, and the apoptosis marker, caspase 3, with high sensitivity and selectivity. |
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Hayat
寻道图强
爱静静
HEIKU
吃不饱星球球长
自由的青亦
SciGPT
OCDer
高大凌寒
从容芮
shinysparrow
银色的喵咪
有人
加湿器
Chloe
cc
乐乐乐乐乐乐
传奇3
Migue
勤劳晓亦
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