| 标题 |
Establishment of fluorescence quantitative RT-PCR method for detecting viremia in piglets immunized with PRRSV vaccines
PRRSV疫苗免疫仔猪病毒血症荧光定量RT-PCR检测方法的建立
相关领域
病毒血症
生物
病毒学
实时聚合酶链反应
接种疫苗
基因
病毒
遗传学
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| 其它 | To establish a method to rapidly detect the duration of viremia in pigs after immunization with porcine reproductive and respiratory syndromevirus(PRRSV)vaccines,according to the PRRSV gene sequence in GenBank,a pair of specific primers for the amplification of N gene were designed,and then the N gene was amplified by RT-PCR and ligated into pMD19-T vector to construct a recombinant plasmid containing the N gene fragment.SYBR GreenⅠdye method was used for quantitative RT-PCR amplification,standard curve was established,and |
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