蛋白激酶C
造血
细胞生物学
生物
细胞分化
同工酶
信号转导
细胞培养
生长因子
激酶
受体
电池类型
分子生物学
细胞
干细胞
生物化学
酶
遗传学
基因
作者
W. Li,Joseph E. Pierce
出处
期刊:Current Topics in Microbiology and Immunology
日期:1996-01-01
卷期号:: 55-65
被引量:5
标识
DOI:10.1007/978-3-642-85232-9_6
摘要
The protein kinase C (PKC) family comprises of a group of serine/threonine kinases that are thought to play a central role in cell growth, differentiation and cellular transformation (for reviews see [1, 2]). To date, more than 10 different PKC isoenzymes have been identified. The differential expression pattern of these isoenzymes in different tissues and cell types implicates that they may be involved in different signaling pathways. This prompted us to individually overexpress different PKC isoenzymes (α,β,δ,ε,ζ, and η) in an interleukin-3 (IL-3)-dependent murine myeloid progenitor cell line, 32D, in order to investigate their possible involvement in mitogenic and differentiation pathways of hematopoietic cells (3). 32D cells express low levels of PKC- δ, - α and - η, as detected by immunoblot analysis using isoenzyme-specific antibodies (3). After overexpression of different PKCs in 32D cells, none of these transfectants demonstrated any morphological changes in the presence of IL-3, and all remained growth factor dependent. However, treatment of 32D cells overexpressing either PKC- α or PKC- δ (32D/ PKC- δ) with 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in pronounced monocytic differentiation, while the parental 32D cells and other PKC isoenzyme transfectants did not readily undergo monocytic differentiation in response to TPA stimulation (3). These data indicate that PKC- α and PKC- δ play a pivotal role in monocytic differentiation and that the activation of certain substrates involved in differentiation can only occur when PKC- α and PKC- δ are activated in the 32D cell system.
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