循环肿瘤细胞
液体活检
间隙
分离(微生物学)
癌症研究
癌症
医学
肿瘤科
生物
内科学
生物信息学
转移
泌尿科
作者
Cäcilia Köstler,Bernhard Polzer,Barbara Alberter
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 11-42
标识
DOI:10.1007/978-1-0716-3621-3_2
摘要
The analysis of circulating tumor cells (CTCs) has shown potential for detection of cancer spread, prognosis, therapeutic target selection, and monitoring of treatment response. CTCs can be obtained repeatedly by simple blood draws as so-called "liquid biopsy." Thus, they can serve as a surrogate material for primary or metastatic tissue biopsies. In addition, isolation of CTCs provides the possibility to investigate those cells which may hold the (molecular) traits responsible for metastatic progression and ultimately patient death. As such, CTCs represent a target of utmost importance in cancer research and therapy. In this chapter, we describe a workflow for the enrichment of CTCs with the FDA-cleared CellSearch® system followed by the isolation of single CTCs using the DEPArray™ technology enabling further molecular single-cell analyses.
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