纳米器件
化学
蛋白质工程
DNA
计算生物学
细胞生物学
生物
生物化学
酶
纳米技术
材料科学
作者
Simona Ranallo,Daniela Sorrentino,Elisabetta Delibato,Gianfranco Ercolani,Kevin W. Plaxco,Francesco Ricci
标识
DOI:10.1002/anie.202115680
摘要
We report here the rational design and optimization of an antibody-responsive, DNA-based device that enables communication between pairs of otherwise non-interacting proteins. The device is designed to recognize and bind a specific antibody and, in response, undergo a conformational change that leads to the release of a DNA strand, termed the "translator," that regulates the activity of a downstream target protein. As proof of principle, we demonstrate antibody-induced control of the proteins thrombin and Taq DNA polymerase. The resulting strategy is versatile and, in principle, can be easily adapted to control protein-protein communication in artificial regulatory networks.
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