单层
液晶
拓扑缺陷
生物物理学
材料科学
形态发生
弹性体
纳米地形
成纤维细胞
组织工程
化学物理
纳米技术
细胞生物学
化学
细胞培养
生物
凝聚态物理
光电子学
复合材料
物理
生物化学
基因
遗传学
作者
Taras Turiv,Jess Krieger,Greta Babakhanova,Hao Yu,Sergij V. Shiyanovskii,Qi‐Huo Wei,Min‐Ho Kim,Oleg D. Lavrentovich
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2020-05-13
卷期号:6 (20)
被引量:103
标识
DOI:10.1126/sciadv.aaz6485
摘要
Eukaryotic cells in living tissues form dynamic patterns with spatially varying orientational order that affects important physiological processes such as apoptosis and cell migration. The challenge is how to impart a predesigned map of orientational order onto a growing tissue. Here, we demonstrate an approach to produce cell monolayers of human dermal fibroblasts with predesigned orientational patterns and topological defects using a photoaligned liquid crystal elastomer (LCE) that swells anisotropically in an aqueous medium. The patterns inscribed into the LCE are replicated by the tissue monolayer and cause a strong spatial variation of cells phenotype, their surface density, and number density fluctuations. Unbinding dynamics of defect pairs intrinsic to active matter is suppressed by anisotropic surface anchoring allowing the estimation of the elastic characteristics of the tissues. The demonstrated patterned LCE approach has potential to control the collective behavior of cells in living tissues, cell differentiation, and tissue morphogenesis.
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