젤라틴을 함유한 생분해성 나노섬유의 제조 및 특성

Biodegradable polymeric nanofibrous matrices containing gelatin were fabricated by simple electrospinning method. The electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV)/gelatin (1:1) nanofiber structure revealed randomly aligned fibers with average diameter of 600 nm determined and calculated by SEM and image analyzer. ATR-FTIR and ESCA mesurement were used to confirm the presence of gelatin in PHBV/gelatin nanofibers. Human fibroblasts` behavior on PHBV/gelatin nanofibrous matrices has been investigated. Fibroblasts were well attached on the surface of control PHBV and PHBV/gelatin nanofibers. Initial cell attachment on PHBV/gelatin nanofibers were significantly higher than that of control PHBV nanofibers, because gelatin has many RGD moiety to mediate cell attachment. Electrospinning of PHBV with gelatin significantly decreased water contact angle of control PHBV nanofiber. Cells could penetrate into the nanofibers and enabled 3D cell culture, due to the hydrophilic nature of PHBV/gelatin nanofibers. PHBV/gelatin nanofibers facilitated spacial distribution, and initial attachment of cells. From the above results, electrospinning of PHBV with gelatin is a promising method for application to cell culture matrices.