生物
造血
祖细胞
干细胞
细胞生物学
转录组
骨髓
多能干细胞
祖细胞
免疫学
脐带血
遗传学
基因表达
基因
作者
Yawen Zhang,Xiaowei Xie,Yaojing Huang,Mengyao Liu,Qiaochuan Li,Jianming Luo,Yunyan He,Xiuxiu Yin,Shihui Ma,Wenbin Cao,Shulian Chen,Jun Peng,Jiaojiao Guo,Wen Zhou,Hongbo R. Luo,Fang Dong,Hui Cheng,Sha Hao,Linping Hu,Ping Zhu,Tao Cheng
标识
DOI:10.1016/j.devcel.2022.11.013
摘要
Hematopoietic stem and progenitor cells (HSPCs) give rise to the blood system and maintain hematopoiesis throughout the human lifespan. Here, we report a transcriptional census of human bone-marrow-derived HSPCs from the neonate, infant, child, adult, and aging stages, showing two subpopulations of multipotent progenitors separated by CD52 expression. From birth to the adult stage, stem and multipotent progenitors shared similar transcriptional alterations, and erythroid potential was enhanced after the infant stage. By integrating transcriptome, chromatin accessibility, and functional data, we further showed that aging hematopoietic stem cells (HSCs) exhibited a bias toward megakaryocytic differentiation. Finally, in comparison with the HSCs from the cord blood, neonate bone-marrow-derived HSCs were more quiescent and had higher long-term regeneration capability and durable self-renewal. Taken together, this work provides an integral transcriptome landscape of HSPCs and identifies their dynamics in post-natal steady-state hemopoiesis, thereby helping explore hematopoiesis in development and diseases.
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