Structure, selenization modification, and antitumor activity of a glucomannan from Platycodon grandiflorum

Platycodon grandiflorum is consumed popularly as a nutritional and healthy plant in East Asia, which has multiple medicinal functions. As an exploration to elucidate the beneficial ingredients, an acetylated glucomannan (PGP40-1) was purified from P. grandiflorum. Structural analysis showed that PGP40-1 was composed of →4)-β-Manp-(1→, →4)-β-Glcp-(1→, →6)-β-Glcp-(1→, and terminal α-Glcp-(1→. PGP40-1 was found to possess weak antitumor activity in vitro, which was thus modified to afford a selenized polysaccharide (Se-PGP40-1) by the HNO3/Na2SeO3 method. Se-PGP40-1 showed significant antitumor activity in cell and zebrafish models, which could inhibit tumor proliferation and migration by inducing cell apoptosis and blocking angiogenesis. The research not only clarifies the ingredients of P. grandiflorum with high economical value, but also affords a potential antitumor agent originating from the plant polysaccharide.