已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Alginate microbeads and hydrogels delivering meropenem and bacteriophages to treat Pseudomonas aeruginosa fracture-related infections

微生物学 抗生素 噬菌体疗法 铜绿假单胞菌 噬菌体 生物膜 抗生素耐药性 美罗培南 化学 抗菌剂 细菌 生物 大肠杆菌 生物化学 遗传学 基因
作者
Baixing Chen,Luis Ponce Benavente,Marco Chittò,Jacek K. Wychowaniec,Virginia Post,Matteo D’Este,Caroline Constant,Stephan Zeiter,Wenli Feng,Mercedes González Moreno,Andrej Trampuž,Jeroen Wagemans,Jolien Onsea,R. Geoff Richards,Rob Lavigne,T. Fintan Moriarty,Willem‐Jan Metsemakers
出处
期刊:Journal of Controlled Release [Elsevier]
卷期号:364: 159-173 被引量:8
标识
DOI:10.1016/j.jconrel.2023.10.029
摘要

Bacteriophage (phage) therapy has shown promise in treating fracture-related infection (FRI); however, questions remain regarding phage efficacy against biofilms, phage-antibiotic interaction, administration routes and dosing, and the development of phage resistance. The goal of this study was to develop a dual antibiotic-phage delivery system containing hydrogel and alginate microbeads loaded with a phage cocktail plus meropenem and evaluate efficacy against muti-drug resistant Pseudomonas aeruginosa. Two phages (FJK.R9–30 and MK.R3–15) displayed enhanced antibiotic activity against P. aeruginosa biofilms when tested in combination with meropenem. The antimicrobial activity of both antibiotic and phage was retained for eight days at 37 °C in dual phage and antibiotic loaded hydrogel with microbeads (PA-HM). In a mouse FRI model, phages were recovered from all tissues within all treatment groups receiving dual PA-HM. Moreover, animals that received the dual PA-HM either with or without systemic antibiotics had less incidence of phage resistance and less serum neutralization compared to phages in saline. The dual PA-HM could reduce bacterial load in soft tissue when combined with systemic antibiotics, although the infection was not eradicated. The use of alginate microbeads and injectable hydrogel for controlled release of phages and antibiotics, leads to the reduced development of phage resistance and lower exposure to the adaptive immune system, which highlights the translational potential of the dual PA-HM. However, further optimization of phage therapy and its delivery system is necessary to achieve higher bacterial killing activity in vivo in the future.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
康阿蛋发布了新的文献求助10
1秒前
777发布了新的文献求助10
3秒前
4秒前
lxy发布了新的文献求助10
5秒前
7秒前
8秒前
不如一默发布了新的文献求助10
9秒前
11秒前
儒雅的焦发布了新的文献求助10
12秒前
star009发布了新的文献求助10
13秒前
Lucas应助康阿蛋采纳,获得10
17秒前
背后的坤完成签到 ,获得积分10
18秒前
18秒前
谷捣猫宁完成签到,获得积分10
20秒前
明昼完成签到,获得积分10
20秒前
所所应助mou采纳,获得10
22秒前
hochorsin发布了新的文献求助10
22秒前
23秒前
余南完成签到 ,获得积分10
24秒前
30秒前
45秒前
46秒前
46秒前
大模型应助丶氵一生里采纳,获得10
47秒前
桐桐应助bukeshuo采纳,获得10
48秒前
ZLXLXX完成签到 ,获得积分10
49秒前
52秒前
53秒前
乐乐应助倒影采纳,获得10
54秒前
夜乡晨发布了新的文献求助10
56秒前
56秒前
虚拟的饼干完成签到,获得积分10
59秒前
1分钟前
1分钟前
酷波er应助rivalsdd采纳,获得30
1分钟前
1分钟前
1分钟前
1分钟前
思源应助虚拟的饼干采纳,获得10
1分钟前
高分求助中
Evolution 10000
ISSN 2159-8274 EISSN 2159-8290 1000
Becoming: An Introduction to Jung's Concept of Individuation 600
Ore genesis in the Zambian Copperbelt with particular reference to the northern sector of the Chambishi basin 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3162031
求助须知:如何正确求助?哪些是违规求助? 2813164
关于积分的说明 7898852
捐赠科研通 2472153
什么是DOI,文献DOI怎么找? 1316366
科研通“疑难数据库(出版商)”最低求助积分说明 631278
版权声明 602129