机械转化
自愈水凝胶
细胞生物学
间充质干细胞
生物医学工程
材料科学
焦点粘着
干细胞
组织工程
细胞分化
明胶
化学
生物
信号转导
生物化学
医学
基因
高分子化学
作者
Keya Ganguly,Sayan Deb Dutta,Aayushi Randhawa,Dinesh K. Patel,Tejal V. Patil,Ki‐Taek Lim
标识
DOI:10.1002/adhm.202202163
摘要
Abstract Biomimetic soft hydrogels used in bone tissue engineering frequently produce unsatisfactory outcomes. Here, it is investigated how human bone‐marrow‐derived mesenchymal stem cells (hBMSCs) differentiated into early osteoblasts on remarkably soft 3D hydrogel (70 ± 0.00049 Pa). Specifically, hBMSCs seeded onto cellulose nanocrystals incorporated methacrylate gelatin hydrogels are subjected to pulsatile pressure stimulation (PPS) of 5–20 kPa for 7 days. The PPS stimulates cellular processes such as mechanotransduction, cytoskeletal distribution, prohibition of oxidative stress, calcium homeostasis, osteogenic marker gene expression, and osteo‐specific cytokine secretions in hBMSCs on soft substrates. The involvement of Piezo 1 is the main ion channel involved in mechanotransduction. Additionally, RNA‐sequencing results reveal differential gene expression concerning osteogenic differentiation, bone mineralization, ion channel activity, and focal adhesion. These findings suggest a practical and highly scalable method for promoting stem cell commitment to osteogenesis on soft matrices for clinical reconstruction.
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