Bioelectrochemical regulation accelerates biomethane production from waste activated sludge: Focusing on operational performance and microbial community

Bioelectrochemical regulation represents a newly emerging strategy to enhance anaerobic digestion (AD) of biowastes. Herein, a novel microbial electrolysis cell (MEC) system, equipped with a pair of carbon brush anode and hybrid Ti/RuO2-graphite felt cathode, was developed to explore the role of bioelectrochemical regulation in the proliferation/enrichment of functional microbes and methanation of waste activated sludge. The methane production was significantly improved by applying bioelectrochemical regulation. The maximum methane yield was 16.4 mL/L-reactor at the applied external voltage 1.2 V and solids retention time 15 d, 8.6-time higher than that of a single AD. Further analysis demonstrated that bioelectrochemical regulation selectively enriched electroactive fermentative partners and methanogens (especially Thermincola, Methanobacterium) in the MEC-AD system and built up a robust syntrophic interaction. This drove the decomposition of complex organics and concurrent bioelectroreduction of CO2 in biogas and subsequently enhanced methane generation. Besides, bioelectrochemical simulation attenuated N2O emissions and enhanced the dewaterability of digested sludge.