多克隆抗体
抗体
免疫学
单克隆抗体
球蛋白
淋巴细胞
免疫荧光
医学
分子生物学
生物
作者
Nathalie Bonnefoy,Claude Vincent,Jean‐Pierre Revillard
出处
期刊:Transplantation
[Ovid Technologies (Wolters Kluwer)]
日期:1991-03-01
卷期号:51 (3): 669-673
被引量:203
标识
DOI:10.1097/00007890-199103000-00024
摘要
Antilymphocyte or antithymocyte globulins were shown to be immunosuppressive when administered to recipients of organ transplants as prophylactic or rescue treatment of acute rejection or in patients with acute graft versus host reactions following bone marrow transplantation. Several monoclonal antibodies specific for activation or adhesion molecules of the T lymphocyte surface can also inhibit experimental or clinical allograft reactions. We have investigated the presence of some antibodies, of defined specificity and documented biological activity, in polyclonal antilymphocyte and antithymocyte globulins in order to get further insight into the mechanism of action of these polyclonal antibodies. Using a quantitative immunofluorescence assay by flow cytometry we could estimate the minimal amounts of antibodies to LFA-1 (CD11a and CD18), CD45, CD3, and CD5. Antibodies to HLA-DR, CD2, CD4, CD8, and CD25 were also demonstrated but could not be quantified. Antibodies to β2-microglobulin were determined by ELISA. These data suggest that interference with functional lymphocyte surface molecules may account at least in part for the immunosuppressive activity of antilymphocyte and antithymocyte globulins.
科研通智能强力驱动
Strongly Powered by AbleSci AI