重编程
生物
诱导多能干细胞
胚胎干细胞
细胞生物学
内胚层
谱系(遗传)
细胞分化
电池类型
细胞
移植
遗传学
基因
医学
外科
作者
Xiang Li,Defang Liu,Yantao Ma,Xiaomin Du,Junzhan Jing,Lipeng Wang,Bingqing Xie,Da Sun,Shaoqiang Sun,Xueqin Jin,Xu Zhang,Ting Zhao,Jingyang Guan,Zexuan Yi,Weifeng Lai,Ping Zheng,Zhuo Huang,Yan‐Zhong Chang,Zhen Chai,Jun Xu,Hongkui Deng
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2017-08-01
卷期号:21 (2): 264-273.e7
被引量:79
标识
DOI:10.1016/j.stem.2017.05.019
摘要
Direct lineage reprogramming, including with small molecules, has emerged as a promising approach for generating desired cell types. We recently found that during chemical induction of induced pluripotent stem cells (iPSCs) from mouse fibroblasts, cells pass through an extra-embryonic endoderm (XEN)-like state. Here, we show that these chemically induced XEN-like cells can also be induced to directly reprogram into functional neurons, bypassing the pluripotent state. The induced neurons possess neuron-specific expression profiles, form functional synapses in culture, and further mature after transplantation into the adult mouse brain. Using similar principles, we were also able to induce hepatocyte-like cells from the XEN-like cells. Cells in the induced XEN-like state were readily expandable over at least 20 passages and retained genome stability and lineage specification potential. Our study therefore establishes a multifunctional route for chemical lineage reprogramming and may provide a platform for generating a diverse range of cell types via application of this expandable XEN-like state.
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