Triclosan uptake and transformation by the green algae Euglena gracilis strain Z

Triclosan is an antimicrobial chemical present in consumer products that is frequently detected in aquatic environments. In this research, we investigated the role of a common freshwater microalgae species, Euglena gracilis for triclosan uptake and transformation in open-water treatment wetlands. Lab-scale wetland bioreactors were created under various conditions of light (i.e., continuous (white) light, red light, and in the dark), media (i.e., wetland, autoclaved wetland, Milli-Q, and growth media water), and presence or absence of algae. Triclosan and its potential transformation products were identified in the water and algae phases. Triclosan transformation occurred most rapidly with reactors that received continuous (white) light, with pseudo first-order rate constants, k, ranging from 0.035 to 0.292 day-1. This indicates that phototransformation played a major role in triclosan transformation during the day, despite light screening by algae. Algae contributed to the uptake and transformation of triclosan in all reactors, and algae and bacteria both contributed to triclosan biotransformation under dark conditions, representative of nighttime conditions. Some transformation products were formed and further transformed, e.g., triclosan-O-sulfate, methoxy and diglucosyl conjugate of hydroxylated triclosan, and dimethoxy and glucosyl conjugate of 2,4-dichlorophenol, suggesting their minimal accumulation over the 25 days of the experiments. This study shows that the combined action of light, microbes, and algae allows the safe transfer and transformation of triclosan in open-water treatment wetlands.