多路复用
化学
同种类的
乳腺癌
检出限
生物传感器
打字
电化学
电极
色谱法
分析化学(期刊)
癌症
内科学
生物化学
计算机科学
语音识别
物理化学
热力学
生物
物理
生物信息学
医学
作者
Ya Zhang,Wenhao Ma,Ning Li,Mei Yang,Changjun Hou,Danqun Huo
标识
DOI:10.1021/acs.analchem.4c01877
摘要
Simultaneous detection of multiple tumor markers is of great significance for an accurate diagnosis and early treatment of cancer. Electrochemical homogeneous biosensing strategies have been shown to have advantages, such as high sensitivity and no electrode modification, but they are still a challenge in the field of simultaneous detection of multiple tumor markers. The ER, PR, HER2, and Ki67 proteins are the standard biomarkers for the clinical molecular typing of breast cancer. Precise, sensitive, and simultaneous detection of these four biomarkers is of great importance in the molecular typing of breast cancer, which helps in the creation of personalized treatment plans. In the present study, we developed an electrochemical homogeneous electrochemical bioplatform based on metal ions/SiO2NPs/magnetic beads for detection of the four biomarkers and simultaneous diagnosis of the 10 types of breast cancer directly in human serum at one system by a single electrode. The electrochemical bioplatform has a short detection time of 140 min; however, the current clinical tissue testing time takes about 1 week. Also, the electrochemical bioplatform selectively detects HER2, ER, Ki67, and PR in a range of 0–1000 pg/mL with detection limits of 2, 1.8, 10.36, and 1.33 pg/mL, respectively.
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