牙周炎
牙槽
基因剔除小鼠
骨吸收
结扎
吸收
医学
分子生物学
化学
内科学
男科
牙科
生物
受体
作者
Hisashi Goto,Takeshi Kikuchi,Yuhei Takayanagi,Yosuke Kamiya,Yuki Suzuki,Shotaro Kawamura,Noritaka Sawada,Jun‐ichiro Hayashi,Akio Mitani
摘要
Abstract Aim To investigate the role of Ebi3‐related cytokines (i.e., interleukin [IL]‐35 and/or IL‐27) in experimental periodontitis using Ebi3 knockout (KO) mice. Materials and Methods The maxillary right second molar teeth of Ebi3 KO mice and C57BL/6 mice were tied with a silk ligature to induce periodontitis. Three days after ligation, gingival tissues were collected for gene expression analyses. Five days after ligation, the maxillae were removed for haematoxylin and eosin staining and immunohistochemistry. Seven days after ligation, the maxillae were removed for micro‐computed tomography. Results The ligated side of Ebi3 KO mice showed intense alveolar bone resorption, which was substantially more pronounced than in wild‐type (WT) mice. IL‐17A expression was significantly higher in the gingiva of the ligated side of Ebi3 KO mice compared with WT mice. IL‐10 expression was significantly lower in Ebi3 KO mice than in WT mice. The ligature‐induced alveolar bone resorption in Ebi3 KO mice that received recombinant IL‐35 injection was significantly less compared with that in Ebi3 KO mice that received control injection. Conclusions Together, these findings suggest that Th17 cells exacerbate experimental periodontitis in mice lacking Ebi3 and that IL‐35 may play a critical role in inhibiting periodontal tissue destruction.
科研通智能强力驱动
Strongly Powered by AbleSci AI