伤害
痛觉过敏
白屈菜红碱
蛋白激酶C
炎症
药理学
医学
坐骨神经
辣椒素
化学
麻醉
内科学
受体
激酶
生物化学
作者
Hui‐Sheng Chen,Jing Lei,Xiang He,Fang Qu,Yang Wang,Weiwei Wen,Hao‐Jun You,Lars Arendt‐Nielsen
出处
期刊:Pain
[Ovid Technologies (Wolters Kluwer)]
日期:2008-03-01
卷期号:135 (1): 31-36
被引量:25
标识
DOI:10.1016/j.pain.2007.04.040
摘要
The roles of central protein kinases A and C (PKA and PKC) in various pain states have intensively been investigated during the past decade. The aim of the present study was to investigate the peripheral involvement of PKA and PKC in persistent nociceptive response, evoked pain behaviors, and inflammation induced by subcutaneous (s.c.) injection of bee venom (BV, 0.2 mg/50 μl) in rats. The effects of intraplantar injection of H-89 (a PKA inhibitor, 5–100 μg/50μl) and chelerythrine chloride (a PKC inhibitor, 5–100 μg/50μl) on BV-elicited persistent nociception (nociceptive flinching reflex), mechanical hyperalgesia, and inflammation were systematically investigated. Pre-treatment with H-89 dose-dependently inhibited only BV-induced mechanical hyperalgesia, but not the persistent nociception and inflammation. In contrast, pre-treatment with chelerythrine chloride dose-dependently inhibited BV-induced sustained nociception and inflammation, but not the mechanical hyperalgesia. Topical pre-treatment of the sciatic nerve with 1% capsaicin significantly blocked the inhibitory effects of the PKC inhibitor on BV-induced inflammation, but not the persistent flinching response. These results indicate that peripheral PKA and PKC involvements in BV-induced pain behaviors differ, and capsaicin-sensitive afferents appear to participate in the pro-inflammatory role of PKC in the BV pain model. Findings from the present study also suggest that targeting specific peripheral protein kinases might prove effective in the treatment of persistent pain and inflammation.
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