乙草胺
异丙甲草胺
化学
荧光团
检出限
核酸酶
免疫分析
荧光
色谱法
胶体金
杀虫剂
纳米颗粒
阿特拉津
纳米技术
DNA
生物化学
抗体
物理
材料科学
免疫学
生物
量子力学
农学
作者
Yonghong Zha,Yansong Li,Pan Hu,Shiying Lu,Hong-Lin Ren,Zeng-Shan Liu,Hualin Yang,Yu Zhou
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2021-10-08
卷期号:93 (41): 13886-13892
被引量:17
标识
DOI:10.1021/acs.analchem.1c02736
摘要
Given the great harm of pesticide residues to the environment and public health, exploring ultrasensitive and low-cost methods for their quantitative analysis becomes intensely necessary. Herein, we proposed a double-functionalized gold nanoparticle (AuNP) probe as a signal amplification immunoassay for the detection of acetochlor (ATC), metolachlor, and propisochlor. The AuNP was modified with IgG and fluorophore-labeled duplex DNA by a polyadenine-based freezing method. The quenched fluorescence can be effectively recovered via duplex-specific nuclease (DSN) with excellent cleaving activity. This approach provided limits of detection (LODs) down to 0.03 ng/mL for ATC, 0.10 ng/mL for metolachlor, 0.14 ng/mL for propisochlor, and 0.08 ng/mL for their mixture. The average recoveries of ATC, metolachlor, and propisochlor were 93.0-106.6% from a corn sample, which are in good agreement with the commercial kit (R2 = 0.9995). This "turn-off" fluorescence immunoassay presents considerable potential in the analysis of chloroacetamide herbicide due to its simple process of probe preparing and ultrahigh sensitivity.
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