异硫氰酸苯乙酯
细胞凋亡
异硫氰酸盐
十字花科蔬菜
癌症研究
程序性细胞死亡
癌症
癌细胞
细胞培养
莱菔硫烷
线粒体
生物
细胞生长
半胱氨酸蛋白酶
化学
细胞生物学
生物化学
遗传学
作者
Nou Ying Tang,Yating Huang,Chun Shu Yu,Yang Ching Ko,Shin Hwar Wu,Bin Ji,Jai Sing Yang,Jinghong Yang,Te Chun Hsia,Ya Yin Chen,Jing Gung Chung
出处
期刊:PubMed
日期:2011-05-01
卷期号:31 (5): 1691-702
被引量:24
摘要
Phenethyl isothiocyanate (PEITC), one of many compounds found in cruciferous vegetables, has been reported as a potential anticancer agent. In earlier studies, PEITC was shown to inhibit cell growth and induction of apoptosis in many cancer cell lines. However, no report has shown whether PEITC can induce apoptosis in human prostate cancer cells. Herein, we aimed to determine whether PEITC has anticancer activity in DU 145 human prostate cancer cells. As a result, we found that PEITC induced a dose-dependent decrease in cell viability through induction of cell apoptosis and cell cycle arrest in the G(2)/M phase of DU 145 cells. PEITC induced morphological changes and DNA damage in DU 145 cells. The induction of G(2)/M phase arrest was mediated by the increase of p53 and WEE1 and it reduced the level of CDC25C protein. The induction of apoptosis was mediated by the activation of caspase-8-, caspase-9- and caspase-3-depedent pathways. Results also showed that PEITC caused mitochondrial dysfunction, increasing the release of cytochrome c and Endo G from mitochondria, and led cell apoptosis through a mitochondria-dependent signaling pathway. This study showed that PEITC might exhibit anticancer activity and become a potent agent for human prostate cancer cells in the future.
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