Experimental Autoimmune Prostatitis: Dihydrotestosterone Influence Over the Immune Response

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Dec 2003Experimental Autoimmune Prostatitis: Dihydrotestosterone Influence Over the Immune Response GUSTAVO P. DISERIO and EDGAR NOWOTNY GUSTAVO P. DISERIOGUSTAVO P. DISERIO and EDGAR NOWOTNYEDGAR NOWOTNY View All Author Informationhttps://doi.org/10.1097/01.ju.0000096680.26488.a1AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: In experimental autoimmune prostatitis in a rat model of chronic prostatic inflammation of noninfectious origin the prostatic 5α-dihydrotestosterone (DHT) concentration decreases because of depressed 5α-reductase activity. This decrease in androgens in situ could favor the development of autoimmune status at the same time. We noted that a DHT increase could protect the gland from immune aggression and/or its consequences in regard to prostatic androgenic metabolism. Materials and Methods: We analyzed in vitro the (3H)-DHT enzymatic bioconversion of prostate homogenates of male accessory sexual gland extract (MAG) immunized rats and MAG immunized plus DHT implanted rats (DSG rats), and performed ventral prostate histological observations. The specific cell immune response against MAG antigen(s) was studied by delayed type hypersensitivity. Results: In DSG and MAG rats, and controls enzymatic activities (3α/3β-hydroxysteroid oxidoreductases) were 112.7 ± 11.3, 91.4 ± 15.0 (not significant) and 147.0 ± 12.8 pmol per minute per mg protein (p <0.025). Histological findings in DSG rat ventral prostates revealed infiltrating mononuclear cell foci in lower quantity and less magnitude than in MAG rat prostates. Delayed type hypersensitivity values were positive in MAG rats and lower in DSG rats in relation to kidney treated and untreated rats. Conclusions: Results suggest that constantly elevated DHT levels could decrease the cell immune response but not at significantly. In contrast, androgenic metabolism remains altered in the presence of exogenous androgens. References 1 : Testosterone metabolism in vitro by prostate homogenate from isoimmunized rats. Cell Mol Biol1983; 29: 245. Google Scholar 2 : 3 Alpha-hydroxy-steroid and 3 beta-hydroxysteroid-oxidoreductase activities in vitro from prostates of isoimmunized rats. Cell Mol Biol1985; 31: 81. Google Scholar 3 : 17 Beta-hydroxysteroid oxidoreductase activity in rat prostate after autosensitization to male accessory sexual glands. Cell Mol Biol1986; 32: 463. Google Scholar 4 : Effect of male accessory glands autoaggression on androgenic cytosolic and nuclear receptors of rat prostate. Cell Mol Biol1992; 38: 201. Google Scholar 5 : Experimental autoimmune prostatitis: in vivo induction of the autoimmune response to lymphocytic soluble factors. Alterations at the endocrine metabolism level. Am J Reprod Immunol1998; 39: 169. Google Scholar 6 : Sex hormones and the immune system. In: Molecular Autoimmunity. Edited by . New York: Academic Press1992: 385. Google Scholar 7 : The influence of testosterone on the development of autoimmune thyroiditis in thymectomized and irradiated rats. Clin Exp Immunol1982; 48: 367. Google Scholar 8 : Approach to the study of the role of sex hormones in autoimmunity. Arthritis Rheum1979; 22: 1170. Google Scholar 9 : Sensitivity to androgen. A possible factor in sex differences in the immune response. Clin Exp Immunol1979; 38: 218. Google Scholar 10 : Positive and negative feedback control by estrogen of luteinizing hormone secretion in the rhesus monkey. Endocrinology1973; 92: 799. Google Scholar 11 : Immune privilege in the anterior chamber of the eye: alloantigens and tumour-specific antigens presented into the anterior chamber simultaneously induce suppression and activation of delayed hypersensitivity to the respective antigens. Immunology1992; 77: 189. Google Scholar 12 : A rapid and sensitive method for the quantitation of migrogram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem1976; 72: 248. Google Scholar 13 : Regulation of prostate growth. J Endocrinol1991; 131: 5. Google Scholar 14 : Time-course study of cellular immune response and testosterone metabolism in an autoimmune model for chronic prostatic inflammation. J Urol1998; 160: 1546. Link, Google Scholar 15 : Experimental autoimmune damage to rat male accessory glands. Am J Reprod Immunol1981; 1: 255. Google Scholar 16 : Experimental autoimmune damage to rat accessory glands. II. T cell requirement in adoptive transfer of specific tissue damage. Am J Reprod Immunol1984; 5: 15. Google Scholar 17 : Histological lesion and testosterone biosynthesis impairment in testes from autoimmune rabbits. Acta Physiol Lat Am1976; 26: 494. Google Scholar 18 : Oxidative stress-related parameters in prostate of rats with experimental autoimmune prostatitis. Prostate1998; 34: 270. Google Scholar 19 : Genetic regulation of testosterone-induced immune suppression. Cell Immunol1987; 104: 91. Google Scholar 20 : The role of interleukin 2 in autoimmunity. Immunol Today1989; 10: 246. Google Scholar From the Catedra de Quimica Biologica Analitica, Facultad de Ciencias Quimicas, Universidad Nacional De Cordoba, Cordoba, Argentina© 2003 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 170Issue 6December 2003Page: 2486-2489 Advertisement Copyright & Permissions© 2003 by American Urological Association, Inc.Keywordsprostatitisstanoloneimmunityrats, WistarprostateandrogensMetricsAuthor Information GUSTAVO P. DISERIO More articles by this author EDGAR NOWOTNY More articles by this author Expand All Advertisement PDF downloadLoading ...