斯氏假单胞菌
纳帕
好氧反硝化
硝酸盐
反硝化
拉伤
化学
氮气
氮平衡
环境化学
核化学
生物化学
生物
有机化学
细菌
反硝化细菌
解剖
遗传学
作者
Bin Zhao,Dan Yang Cheng,Pan Tan,Qiang An,Jin Guo
标识
DOI:10.1016/j.biortech.2017.11.038
摘要
An aerobic denitrifier was newly isolated and identified as Pseudomonas stutzeri strain XL-2. Strain XL-2 removed 97.9% of nitrate with an initial concentration about 100 mg/L. Nitrogen balance indicates that 12.4% of the initial nitrogen was converted to N2O, and 62.4% was converted to N2. Single factor experiments indicate that the optimal conditions for nitrate removal were C/N ratio of 10, temperature of 30 °C and shaking speed of 120 rpm. Sequence amplification indicates that the denitrification genes of napA, nirS, norB and nosZ were present in strain XL-2. Combined with nitrogen balance, strain XL-2 presents the metabolic pathway of NO3- → NO2- → NO → N2O → N2 under aerobic conditions. The expression of napA and nirS might be responsible for the tolerance of dissolved oxygen by strain XL-2 during denitrification process.
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