偶氮甲烷
结直肠癌
结肠炎
发病机制
炎症性肠病
医学
大肠癌小鼠模型的建立
肠道菌群
胃肠病学
腺癌
内科学
疾病
免疫学
癌症
作者
Yaguang Li,Junhong Liu,Gaixia Liu,Zhenhong Pan,Mingxia Zhang,Yao Ma,Qingxia Wei,Hongping Xia,Rui Xue Zhang,Junjun She
摘要
The human appendix has been recently implicated to play important biological roles in the pathogenesis of various complex diseases, such as colorectal cancer, inflammatory bowel disease, and Parkinson’s disease. To study the function of the appendix, a gut disease-associated murine appendectomy model has been established and its step-by-step protocol is described here. This report introduces a facile protocol for caecal patch removal in mice followed by the chemical induction of chronic colitis-associated colorectal cancer using a combination of dextran sulfate sodium (DSS) and azoxymethane (AOM). IgA specific cells and IgA concentration were significantly reduced upon removal of the caecal patch in male C57BL/6 mice compared to those in the sham group. Simultaneously administering 2% DSS and AOM resulted in nearly 80% mice survival in both sham and appendectomy groups without significant body weight loss. Histological results confirmed colonic inflammation and different degrees of adenocarcinoma. This model can be used for the study of the functional role of the appendix in maintaining gut microbiota homeostasis and pathogenesis of gut colitis and malignancies, as well as for the potential development of drug targeting therapies.
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